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qPCR is fairly straightforward to execute as compared to movement cytometry that needs substantial know-how for reproducible results. In fact, move cytometric enumeration of microbial cells was in the beginning limited to pure cultures [31] and nevertheless stays complicated when carried out in complicated matrices [32].

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The sequencing reads assigned to distinct taxa in Each and every sample ended up divided by the entire number of reads for that sample to acquire relative abundances of the taxa in Every sample. The relative abundances acquired according to the sequencing reads ended up translated into total abundances by multiplying the relative abundance of each and every taxon by the full bacterial abundance inside the sample.

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